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Clinical Chemistry 1: 317-323, 1955;
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Clinical Chemistry, Vol 1, 317-323, Copyright © 1955 by the American Association for Clinical Chemistry

Estimation of Lipid Fractions of Blood Plasma

System Usingthe Allen Volumetric Method

Andre C. Kibrick 1 and S. J. Skupp 1

1 Clinical Laboratories of the New York Veterans Administration Hospital in affiliation with New York University College of Medicine.

The Allen volumetric method for plasma fat has been modified by calibration to include the percentage of olive-oil recovery. The values obtained from calibration with mercury are corrected for the 125 per cent recovery of the oil. The validity of the entire procedure has been discussed.

A simple system of estimating the lipid fractions of plasma has been described. Total lipids are the total of Allen fat + (25 x lipid P). Neutral fat is Allen fat-cholesterol-cholesterol fatty acids. Total fatty acids are X neutral fat + cholesterol fatty acids + (0.64 x phospholipids).

The values of total fatty acids have been compared with those obtained by oxidation with dichromate and by determining the esterified fatty acids with iron perchlorate.

The values of neutral fat found in 13 apparently normal individuals are shown to range from 0 to 88 and to give an average value of 28 mg./100 ml.

Submitted on April 25, 1955






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Copyright © 1955 by the American Association for Clinical Chemistry.