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Clinical Chemistry 10: 1-12, 1964;
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Clinical Chemistry, Vol 10, 1-12, Copyright © 1964 by the American Association for Clinical Chemistry

Fluorometric Determination of Urinary Metanephrine and Normetanephrine

Shannon Brunjes 1, Donald Wybenga 1, and Varner J. Johns Jr. 1

1 Department of Medicine, School of Medicine, Loma Linda University and the Los Angeles County Hospital, Los Angeles, Calif.

A detailed procedure for the quantitative determination of metanephrine and normetanephrine is given. Epinephrine and norepinephrine are eliminated by selective adsorption on an alumina column. Metanephrine and normetanephrine are isolated with a cation-exchange resin. Fluorescence is developed by a two-step ferricyanide oxidation at two different pH values followed by tautomerization. The concentrations of both metanephrine and normetanephrine are quantitated by differential fluorometry.

Submitted on July 19, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.