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Clinical Chemistry 10: 75-82, 1964;
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Clinical Chemistry, Vol 10, 75-82, Copyright © 1964 by the American Association for Clinical Chemistry

Automated Determination of Serum Alkaline Phosphatase Using a Modified Bodansky Technic

H. Keay 1 and J. A. Trew 2

1 Department of Laboratories, Regina General Hospital, Regina, Sask.
2 Department of Biochemistry, University of Saskatchewan, Saskatoon,. Sask., Canada.

An automated procedure for the measurement of alkaline phosphatase activity by a modification of the Bodansky method is described. It has been possible to adapt the Fiske-SubbaRow method for phosphate so that alkaline phosphatase is determined by a second run, immediately following the determination of inorganic phosphate.

Studies of the effect of time, pH, and concentration of barbital on the enzyme activity are discussed, and the advantages of the method are listed.

Submitted on March 30, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.