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Clinical Chemistry 10: 869-890, 1964;
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Clinical Chemistry, Vol 10, 869-890, Copyright © 1964 by the American Association for Clinical Chemistry

Application of Atomic Absorption Spectrophotometry in the Determination of Calcium in Serum

Alfred Zettner 1 and David Seligson 1

1 Clinical Laboratories, Department of Medicine and Department of Pathology, Yale University Medical Center, New Haven, Conn.

Atomic absorption spectrophotometry (AAS) was applied to the determination of serum calcium. A special diluent was developed to abolish the effect of absorption depressors. This made possible measurement of calcium in directly diluted serum samples without prior removal of any serum constituents. Values obtained by AAS on calcium oxalate precipitates from serum were identical with those found by the direct dilution technique.

Submitted on October 21, 1963




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Copyright © 1964 by the American Association for Clinical Chemistry.