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Clinical Chemistry 10: 907-917, 1964;
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Clinical Chemistry, Vol 10, 907-917, Copyright © 1964 by the American Association for Clinical Chemistry

A Photometric System for Automatically Reading Concentration

D. K. McKay 1, D. Seligson 1, and W. R. Jewett 1

1 Department of Clinical Laboratories, Grace-New Haven Community Hospital, and Departments of Medicine and Pathology, Yale University School of Medicine, New Haven, Conn.

Spectrophotometric or colorimetric data are normally obtained in some type of photometric unit and must be dealt with mathematically to provide a final result expressed in concentration. Such mathematical operations are time-consuming, require data transfer, and are subject to human error. A photometric system has been devised which can be adjusted to perform calculations automatically and provide direct readout of concentration. This system combines logarithmic conversion of %T with control of both m and b constants in the straight-line equation, y = mx + b, in which y is absorbance, m is slope, x is concentration, and b is the blank correction. The system also provides linearizing compensation for those reactions which do not follow the Beer-Lambert law. The calibration procedure for the system is described, as is the linearization of nonlinear standard curves.

Submitted on October 21, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.