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Clinical Chemistry 10: 1002-1006, 1964;
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Clinical Chemistry, Vol 10, 1002-1006, Copyright © 1964 by the American Association for Clinical Chemistry

The Estimation of Galactose in Plasma Using Galactose Oxidase

J. D. Ford 1 and J. C. Haworth 2

1 Department of Clinical Investigation, Children's Hospital, Winnipeg, and the Department of Paediatrics, University of Manitoba, Canada.
2 The Winnipeg Clinic, Winnipeg 1, Manitoba, Canada.

The estimation of galactose in plasma by the enzyme system galactose oxidase-peroxidase was investigated. The method was found to be relatively specific for galactose although xylose and ascorbic acid reacted to a slight degree with the enzyme; lactose also reacted with the enzyme, probably because partial hydrolysis of the disaccharide released galactose. The relationship between absorbance and concentration of galactose greater than 20 mg./100 ml. was linear. Below this value the line curved toward the origin, making accuracy uncertain unless a number of aqueous standards can be run with each batch of estimations.

Submitted on July 5, 1963
Accepted on October 8, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.