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Clinical Chemistry 10: 1025-1027, 1964;
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Clinical Chemistry, Vol 10, 1025-1027, Copyright © 1964 by the American Association for Clinical Chemistry

Interference of Thiouracil in the Ferric Chloride-Sulfuric Acid Cholesterol Reaction

Eugene W. Rice 1

1 Department of Biochemistry, The William H. Singer Memorial Research Laboratory of the Allegheny General Hospital, Pittsburgh, Pa.

Thiouracil, previously recommended as a preservative to be added to serum for butanol-extractable iodine determinations, inhibits the ferric chloride-sulfuric acid color reaction for cholesterol. The color inhibition may be eliminated by shaking the serum for about 5 min. with solid silver iodate prior to cholesterol analysis.

Submitted on November 19, 1963
Accepted on December 9, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.