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Clinical Chemistry 10: 1028-1038, 1964;
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Clinical Chemistry, Vol 10, 1028-1038, Copyright © 1964 by the American Association for Clinical Chemistry

Spectrophotometric Determination of Magnesium in Blood Serum Using Magon

Patricia J. Burcar 1, Albert J. Boyle 1, and Robert E. Mosher 1

1 Department of Research, Providence Hospital, Detroit 8, Mich. (P.J.B. and R.E.M.), and the Department of Research, Providence Hospital and Department of Chemistry, Wayne State University, Detroit 8, Mich. (A.J.B.).

A spectrophotometric method for the determination of serum magnesium using Magon, 1-azo-2-hydroxy-3- (2,4-dimethylcarboxanilido) naphthalene- 1’- (2-hydroxybenzene), is presented. The serum is prepared by a nitric-perchloric acid digestion. Interfering phosphate is removed as aluminum phosphate. The colored complex is developed at pH 11.2 in an alcoholic medium and read at 505 mµ.

Submitted on August 5, 1963
Accepted on December 10, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.