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Clinical Chemistry 10: 986-990, 1964;
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Clinical Chemistry, Vol 10, 986-990, Copyright © 1964 by the American Association for Clinical Chemistry

Sialic Acid (N-Acetylneuraminic Acid) in Human Serum

J. A. Cabezas 1 and J. Vazquez Porto 1

1 Chair of Biochemistry, Faculty of Pharmacy, University of Santiago de Compostela, Spain.

A simple spectrophotometric method is described for the sialic acids which utilizes the reaction with resorcinol and extraction with butyl acetate-n-butanol as a solvent. Normal values for adults, utilizing N-acetylneuraminic acid as a standard, were found to be 60 ± 10.4 mg./100 ml. Values for newborn babies averaged 40.4 ± 5.7. Similar values were found for specimens from 2-month-old infants to 27-year-old adults. Higher values were noted in the case of rickets, severe burns, rheumatic disease, and tuberculosis.

Submitted on December 19, 1961






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Copyright © 1964 by the American Association for Clinical Chemistry.