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Clinical Chemistry 10: 1121-1130, 1964;
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Clinical Chemistry, Vol 10, 1121-1130, Copyright © 1964 by the American Association for Clinical Chemistry

Application and Modification of the Momose-Ohkura Fluorometric Determination of Blood Glucose

Bernadette B. Bourne 1

1 Departments of Neurology and Biochemistry, University of Miami, School of Medicine, Miami 36, Fla.

Results of the fluorometric determination of normal and abnormal blood glucose by the method of Momose and Ohkura (2) are lower in many instances than those obtained by reduction methods. The sensitivity of the method was greatly increased by readings at an activation of 470 mµ, a previously unreported maximum for the reaction product, in 20% sulfuric acid. A modified method is presented which uses a 1:1000 dilution of 2 µl. of blood. Protein-free supernate equivalent to 0.5 µl. of blood is reacted. Final volume of fluorophore is 10 ml.

Accepted on March 13, 1964






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Copyright © 1964 by the American Association for Clinical Chemistry.