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Clinical Chemistry 10: 103-115, 1964;
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Clinical Chemistry, Vol 10, 103-115, Copyright © 1964 by the American Association for Clinical Chemistry

A Modified Procedure for the Estimation of Total 17-Hydroxycorticosteroids (Porter-Silber Chromogens) in Urine

John H.C. Liu 1

1 Box 36329, Houston 36, Tex.

A modified procedure for the determination of urinary 17-hydroxycorticosteroids (di-hydroxy-acetonyl-corticoids) is described. The use of ethyl ether in a single extraction step and the stabilization of the phenylhydrazine-sulfuric acid reagent with sodium chloride are the unique features of the method. Studies of chromogenicities of steroids, recovery, pH effect on extraction, and salt effect on the stability of Porter-Silber reagent in the presence and absence of light during storage are reported; chemical aspects of chromogenic development of various steroids are discussed, clinical ranges compared, and interpretations evaluated.

Submitted on September 25, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.