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Clinical Chemistry 10: 128-135, 1964;
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Clinical Chemistry, Vol 10, 128-135, Copyright © 1964 by the American Association for Clinical Chemistry

Quantitation of Serum Urea as Microcapillary Columns of Dixanthylurea

R. L. Searcy 1, J. L. Korotzer 1, G. L. Douglas 1, and L. M. Bergquist 1

1 Departments of Pathology, Los Angeles County General Hospital (Unit 2), California College of Medicine, Los Angeles, Calif.

A simple technic is proposed for direct estimation of urea in terms of columns of dixanthylurea precipitated from serum. The procedure is reproducible and yields results comparable to those obtained colorimetrically using normal and uremic sera. An equation has been derived which permits conversion of levels of dixanthylurea columns, in millimeters, to urea nitrogen concentrations. The new method is useful in detecting as well as monitoring cases of uremia.

Submitted on July 12, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.