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Clinical Chemistry 10: 159-169, 1964;
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Clinical Chemistry, Vol 10, 159-169, Copyright © 1964 by the American Association for Clinical Chemistry

Simultaneous Determination of Calcium and Magnesium of Serum by a Single Chelometric Titration

Sanford H. Jackson 1 and Florence Brown 1

1 Research Institute of the Hospital for Sick Children, Toronto, Ontario, Canada.

Titration of a trichloracetic acid filtrate of serum with EDTA is carried out at pH 9.8 using an excess of Calmagite indicator. Successive photometric readings of percentage transmission, made with an EEL titrator, are plotted against the volume of EDTA added. There is very little change at first while calcium is being complexed; the reading then decreases rapidly while magnesium is being titrated, followed by a flattening of the curve at completion of the titration. Hence, two sharp "breaks" are obtained in the titration curve, the first corresponding to the amount of calcium and the second to the amount of magnesium.

Submitted on November 28, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.