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Clinical Chemistry 10: 191-196, 1964;
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Clinical Chemistry, Vol 10, 191-196, Copyright © 1964 by the American Association for Clinical Chemistry

An Improved Method for the Measurement of Urinary 17-Ketosteroids of Small Laboratory Animals

W. T. Ryan 1, W. F. Ferro 1, and A. L. Beyler 1

1 Sterling-Winthrop Research Institute, Rensselaer, N. Y.

A modified procedure for the estimation of urinary 17-ketosteroids includes the addition of formalin to urine before hot-acid hydrolysis and a separation of Zimmerman chromogen from nonspecific chromogen. Values of 17-ketosteroids are reported for the castrate rat and the intact guinea-pig following ACTH administration.

Submitted on October 4, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.