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Clinical Chemistry 10: 197-213, 1964;
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Clinical Chemistry, Vol 10, 197-213, Copyright © 1964 by the American Association for Clinical Chemistry

Estimation of Bilirubin Using Acetamide

Joseph H. Boutwell Jr. 1

1 Biochemistry Department, Temple University School of Medicine and Temple University Hospital, Philadelphia, Pa.

A study of the effect of acetamide as an accelerator of the reaction between free bilirubin and diazotized sulfanilic acid (DSA) has led to its use in a method for serum bilirubin which is simple, sensitive, linear over a wide range, and which requires neither excessive dilution nor protein precipitation. The conditions necessary for greatest sensitivity and for linearity have been established with regard to the pH of coupling and photometry, the concentration of DSA, the minimizing of turbidities, and the effect of protein.

Submitted on October 15, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.