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Clinical Chemistry 10: 281-290, 1964;
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Clinical Chemistry, Vol 10, 281-290, Copyright © 1964 by the American Association for Clinical Chemistry

Determination of Free and Total Indole-3-acetic Acid and of the Indole Index

J. Fischl 1 and S. Rabiah 1

1 Department of Biochemistry, the "Asaf Harofe" Government Hospital, Zerifin, Israel.

A new method is described for measurement of the urinary excretion of free and total indole-3-acetic acid and for obtaining the chromogenic value of the total indoles. The color reaction employed is Fischl's (1) tryptophan reaction. Hydrolysis and extractions were done by Weissbach's (2) procedure. The ratio of the various fractions is expressed by a new term: "indoles index" (I.I.). By the evaluation of the data obtained under various conditions, many abnormalities of tryptophan metabolism may be detected.

Submitted on September 29, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.