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Clinical Chemistry 10: 321-334, 1964;
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Clinical Chemistry, Vol 10, 321-334, Copyright © 1964 by the American Association for Clinical Chemistry

Photometric Determination of Urinary Proteins

Abraham Saifer 1 and Shirley Gerstenfeld 1

1 Biochemistry Department, Isaac Albert Research Institute of the Jewish Chronic Disease Hospital, Brooklyn 3, N. Y.

Previous reports have dealt with application of a tryptophan method for determination of serum total (19) and gamma (20) globulins and CSF total globulins (21). The method has now been extended to the determination of urinary globulins.

Total urinary protein (including mucoprotein) was determined with the Lowry procedure after perchloric acid-acetone precipitation. Normal values on morning samples (32 subjects) are: T.P., 24.0 ± 19.6 mg./100 ml.; protein (albumin plus globulin), 6.9 ± 4.3 mg./100 ml.; mucoprotein, 17.1 ± 16.3; globulin, 4.4 ± 2.4, albumin, 2.5 ± 2.4; and A/G ratio, 0.58 ± .4 S.D. Preliminary data on patients with a variety of kidney ailments indicate that an increase in the urinary globulin fraction is a sensitive indicator of early renal damage.

Submitted on February 26, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.