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Clinical Chemistry 10: 381-390, 1964;
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Clinical Chemistry, Vol 10, 381-390, Copyright © 1964 by the American Association for Clinical Chemistry

Determination of Exogenous Galactose in Blood using "Specially Purified" Glucose Oxidase

Sheldon S. Waldstein 1, Alvin Dubin 1, Albert Newcomer 1, and Charles H. McKenna 1

1 Department of Medicine, Northwestern University Medical School, and the Hektoen Institute for Medical Research of the Cook County Hospital, Chicago, Ill.

A method is described for the determination of exogenous galactose in blood by a modification of the Nelson-Somogyi hexose procedure in which glucose oxidase is used to eliminate interfering glucose. The use of "specially purified" glucose oxidase (SPGO) achieves low, regular blanks and satisfactory replicates, and enhances accuracy, in contrast to the results obtained with crude enzyme preparations. The ready availability of SPGO makes the method satisfactory and practical for the clinical laboratory.

Submitted on April 6, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.