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Clinical Chemistry 10: 417-423, 1964;
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Clinical Chemistry, Vol 10, 417-423, Copyright © 1964 by the American Association for Clinical Chemistry

Analyses on Heat-coagulated Blood and Serum

II. The Determination of Serum Inorganic Phosphate

Morris London 1 and Jesse H. Marymont Jr. 2

1 Division of Laboratories, North Shore Hospital, Manhasset, Long Island, N. Y.
2 Department of Laboratories, Wesley Medical Center, Wichita 14, Kans.

A method is described that does not employ chemical precipitation for the quantitative separation of serum inorganic phosphate from the proteins present. The separation is accomplished by heat coagulation of serum and subsequent extraction of phosphate from the intact clot with dilute sulfuric acid. Serum (0.25 ml.) is placed at the bottom of a 25x95-mm. shell vial and coagulated in a boiling-water bath for 2 min. The resultant coagulum is firm, adherent to the container, and not easily disrupted; 6.0 ml. of O.3N H2SO4 is then added to cover the coagulum, and the phosphate extracted. The phosphate in the extract is determined by a phosphomolybdate reduction procedure.

Submitted on October 1, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.