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Clinical Chemistry 10: 479-489, 1964;
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Clinical Chemistry, Vol 10, 479-489, Copyright © 1964 by the American Association for Clinical Chemistry

A Microprocedure for the Determination of 4-Pyridoxic Acid in Urine

Muriel J. Woodring 1, Dorothy H. Fisher 1, and Clara A. Storvick 1

1 Nutrition Research Laboratory, School of Home Economics and the Agricultural Experiment Station, Oregon State University, Corvallis, Ore. (Technical Paper No. 1644)

A microprocedure for the estimation of urinary 4-pyridoxic acid which is apparently specific, yet simple enough for routine clinical use, has been adapted from the Fujita et al. (16-17) and Reddy et al. (22) procedures. Average values for urinary 4-pyridoxic acid for 7 women ranged from 3.8 to 6.9 µM/24 hr. by this method. These values are compatible with average daily intakes of vitamin B6 and indicate that by this method interfering fluorescent substances have been removed by chromatography. Good agreement was obtained with replicate analyses.

Submitted on February 11, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.