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Clinical Chemistry 10: 509-518, 1964;
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Clinical Chemistry, Vol 10, 509-518, Copyright © 1964 by the American Association for Clinical Chemistry

A Rapid Semiautomatic System of Chemical Analysis using True Microspecimens

A. Richard Walter 1 and Horace W. Gerarde 1

1 Medical Research Division, Esso Research and Engineering Co., Linden, N. J., and the Bureau of Biological Research, Rutgers University, New Brunswick, N. J.

This paper describes some biochemical applications of a self-filling, self-measuring, disposable, dilution micropipet. The device consists of a precision capillary in a plastic adapter and an attachable plastic bottle containing a premeasured volume of diluent or reagent. The human error in measuring microvolumes of liquid is elimimated by the self-filling, self-measuring action of the capillary. A quantitative transfer of sample to reagent is made by introducing the capillary into the bottle and rinsing the capillary by applying and releasing pressure on the walls of the container. The volume and composition of the reagent in the bottle can be varied depending on the test to be performed. An interesting application is its use as a disposable centrifuge tube containing a premeasured volume of stable deproteinizing agent. Other examples are determination of protein, bilirubin, serum sodium, and potassium, and predilution of serum or plasma for the microglucose determination using automatic chemical analysis.

Submitted on December 21, 1962






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Copyright © 1964 by the American Association for Clinical Chemistry.