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Clinical Chemistry 10: 575-580, 1964;
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Clinical Chemistry, Vol 10, 575-580, Copyright © 1964 by the American Association for Clinical Chemistry

A New Method for the Determination of Ascorbic Acid in Urine

Lee Kum-Tatt 1 and P. C. Leong 1

1 Clinical Biochemistry Section, Department of Pathology, Sepoy Lines, Singapore-3.

A simple method for the determination of ascorbic acid in urine was developed. The method is based on the quantitative reduction of mercuric chloride to mercurous chloride at pH 3.5-5.0. The insoluble mercurous chloride was separated by centrifugation, dissolved in excess standard iodine-potassium iodide solution and back-titrated with standard sodium thiosulphate. The method has precision, and as little as 1.0 mg. of ascorbic acid per 100 ml. of urine can be determined with an accuracy of ± 3%. Common substances found in urine do not interfere.

Submitted on May 25, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.