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Clinical Chemistry 10: 673-677, 1964;
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Clinical Chemistry, Vol 10, 673-677, Copyright © 1964 by the American Association for Clinical Chemistry

Enzymatic Method for Determination of Vanillyl Mandelic Acid

Carmen L. Rosano 1

1 General Medical Research Laboratory, VA Hospital, Albany, N. V. 12208.

An enzymatic method is described for the measurement of vanillyl mandelic acid (VMA). The method utilizes an L-mandelic acid dehydrogenase preparation from Pseudomonas fluorescens A-312 to catalyze the transformation of VMA to vanillin. The method is specific for VMA because only the final product, vanillin, will absorb maximally at 350 mµ. The results compare favorably with the method of Pisano et al.(2).

Submitted on July 18, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.