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Clinical Chemistry 10: 726-729, 1964;
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Clinical Chemistry, Vol 10, 726-729, Copyright © 1964 by the American Association for Clinical Chemistry

A Stable Murexide Reagent for the Estimation of Calcium in Micro Quantities of Serum

Peter D. Spare 1

1 Department of Pathology, Sudbury Memorial Hospital, Sudbury, Ont., Canada.

The effects of various solvents on the fading rate of alkaline murexide were studied. Glycerol, methanol, and propylene glycol were found to retard the fading rate of murexide at pH 12.0 to a considerable extent. Propylene glycol was the most satis-factory of these solvents. It was further noted that the fading rate of alkaline murexide in propylene glycol-water solution was directly proportional to the amount of water contained in the reagent, and that a reagent stable for at least 1 week at room temperature could be prepared.

Submitted on August 19, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.