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Clinical Chemistry 10: 767-780, 1964;
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Clinical Chemistry, Vol 10, 767-780, Copyright © 1964 by the American Association for Clinical Chemistry

Complexometric Titration of Calcium and Magnesium by a Semiautomated Procedure

James D. Jones 1 and Warren F. McGuckin 1

1 Section of Biochemistry, Mayo Clinic and Mayo Foundation, Rochester, Minn.

A semiautomatic procedure for the direct estimation of both serum calcium and magnesium is described. The indicator used, Eriochrome blue SE (EBSE), has the advantage of stability and freedom from interference by naturally occurring substances. The procedure requires 3-5 min. per sample and is very reproducible. The values obtained agree with those obtained by back-titration of excess chelator and are consistently 0.3 mg./100 ml. less than those obtained by a standard oxalate precipitation method.

Submitted on September 3, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.