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Clinical Chemistry 10: 793-798, 1964;
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Clinical Chemistry, Vol 10, 793-798, Copyright © 1964 by the American Association for Clinical Chemistry

Use of Halogenated Acetates and Formaldehyde as Plasma Additives in Inhibiting Heat Denaturation of Plasma Proteins

Donald Dawson 1, Rostik Zajtchuk 2, and J. Garrott Allen 2

1 Pontiac General Hospital, Pontiac, Mich.
2 William H. Danforth Laboratory for Research in Surgery, Department of Surgery, University of Chicago, Chicago 37, Ill.

Halogenated acetates alone and in combination with dextrose and formaldehyde will protect against heat denaturation of plasma proteins even when plasma is heated for 10 hr. at 60°. Application of this principle would afford reclamation of plasma from outdated blood bank blood.

Changes in protein patterns were observed by paper electrophoresis. Other measurements of denaturation were pH, absorbance at 610 mµ, and viscosity. The chemical reaction between the additives and plasma are considered, and estimations of toxicity are given.

Submitted on March 7, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.