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Clinical Chemistry 10: 845-852, 1964;
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Clinical Chemistry, Vol 10, 845-852, Copyright © 1964 by the American Association for Clinical Chemistry

Spectrophotometric Determination of Microgram Quantities of Copper in Biologic Materials

Ramon E. Stoner 1 and Waldemar Dasler 1

1 Department of Biochemistry, The Chicago Medical School, Chicago 12, Ill.

A procedure for the determination of microgram quantities of copper in biologic material is described. The sample is wet-ashed with a nitric acid-perchloric acid mixture. The copper is extracted from an alkaline solution of the ash with a solution of diphenylcarbazide in benzene. Absorbance of the red complex is read at 540 mµ. The copper in as little as 0.5 ml. of serum can be determined and the method is suitable for routine determinations. The use of microcells makes possible the analysis of 0.1 ml. of serum.

Submitted on November 1, 1963






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Copyright © 1964 by the American Association for Clinical Chemistry.