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Clinical Chemistry 11: 413-421, 1965;
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Clinical Chemistry, Vol 11, 413-421, Copyright © 1965 by the American Association for Clinical Chemistry

Coulometric Determination of Protein Nitrogen

Application to Direct Titration of Kjeldahl Digests

Gary D. Christian 1, Edward C. Knoblock 1, and William C. Purdy 1

1 Division of Biochemistry, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012, and Department of Chemistry, University of Maryland, College Park, Md.

A coulometric method is described for the determination of ammonia resulting from micro-Kjeldahl digestion of protein samples. The method has been applied to the direct titration of the ammonia in the digests. Routine titration of the ammonia resulting from 10-µl. serum samples is described; the method has been applied to 1-µl. samples. Results are compared with standard macro- and micro-Kjeldahl procedures.

Submitted on May 19, 1964
Accepted on September 15, 1964






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Copyright © 1965 by the American Association for Clinical Chemistry.