Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 12: 1-17, 1966;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hamilton, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hamilton, R. H.

Clinical Chemistry, Vol 12, 1-17, Copyright © 1966 by the American Association for Clinical Chemistry

A Direct Photometric Method for Chloride in Biological Fluids, Employing Mercuric Thiocyanate and Perchloric Acid

Robert Houston Hamilton 1

1 Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pa. 19140.

The reagent described is 5.5 M in perchloric acid and 3.3 M in urea. It contains ferric ions, mercuric thiocyanate, mercuric ions (from mercuric perchlorate), and mercuric chloride. Serum dissolves directly in this reagent to yield a clear, reddish solution.

When chloride ions are added, they combine first with the free mercuric ions, and then with some of the mercuric ions from the mercuric thiocyanate. Liberated thiocyanate combines with ferric ions to yield red ferric thiocyanate. The color is much more intense in the presence of strong perchloric acid than in other aqueous acid mixtures, Its intensity can be regulated at will by changing the concentration of the ferric iron. The presence of mercuric chloride in the reagent improves linearity between absorbance and chloride concentration.

After the total absorbance is determined, compensation for absorbance by other substances is secured by adding mercuric ions to the photometer tube to reverse the color-producing reaction of chloride, reading the residual absorbance, and subtracting it from the total absorbance, to give a net absorbance produced by chloride alone.

Submitted on July 15, 1965
Accepted on October 28, 1965






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1966 by the American Association for Clinical Chemistry.