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Clinical Chemistry 12: 659-669, 1966;
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Clinical Chemistry, Vol 12, 659-669, Copyright © 1966 by the American Association for Clinical Chemistry

Use of Constant Temperature and Sodium Borohydride in the Trihydroxyindole Method for Catecholamines

Elizabeth Carlsen Gerst 1, Odd S. Steinsland 1, and William W. Walcott 1

1 Department of Physiology, College of Physicians and Surgeons, Columbia University, New York, N. Y. 10032.

Small temperature variations during fluorescent analysis with the trihydroxyindole method can introduce significant errors in the measurement of 15µg./L. or less of epinephrine or norepinephrine. To minimize temperature differences, we have devised a simple, constant-temperature system for the Farrand fluorometer.

Decomposition of ascorbic acid prior to and during analysis also must be prevented. Sodium borchydride was found to be an effective stabilizing agent that contributes negligible fluorescence.These modifications minimize or eliminate much of the difficulty previously encountered in the use of the trihydroxyindole method.

Submitted on January 10, 1966
Accepted on May 21, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.