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Clinical Chemistry 12: 701-708, 1966;
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Clinical Chemistry, Vol 12, 701-708, Copyright © 1966 by the American Association for Clinical Chemistry

Phenolphthalein Monophosphate as a Substrate for Serum Alkaline Phosphatase

An Appraisal

J. H. Wilkinson 1 and Ann V. Vodden 1

1 Pepper Laboratory of Clinical Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pa. 19104.

The procedure for serum alkaline phosphatase determination employing phenolphthalein monophosphate as substrate was evaluated under routine clinical laboratory conditions. A straight-line response to increasing serum concentration was observed, and the reaction was shown to be Mg++ dependent. The method proved to be extremely simple, and the results obtained correlated well with those given by a standard routine procedure involving determination of the liberated inorganic phosphate.

Submitted on March 4, 1966
Accepted on June 20, 1966







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Copyright © 1966 by the American Association for Clinical Chemistry.