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Clinical Chemistry 12: 144-150, 1966;
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Clinical Chemistry, Vol 12, 144-150, Copyright © 1966 by the American Association for Clinical Chemistry

The Determination of Amino Acids in 0.1 ml. of Blood Plasma

E. McEvoy-Bowe 1 and S. Sarojini Thevi 1

1 Department of Biochemistry, University of Singapore, Singapore 3.

A method has been developed for the determination of the following amino acids in 0.1 ml. of blood plasma: alanine, arginine, glutamine, glycine, lysine, serine, taurine, threonine, and valine. A micro Sephadex G-25 column was used to separate the plasma proteins from the amino acids. The amino acid fraction, which is collected in 1 ml. of eluate, is then desalted in an electrolytic desalter, and lyophilized. The residue is dissolved in 0.2 ml. 0.1N HCI, and 50 µl. is applied to paper for 2-dimensional chro matography. The estimation of the amino acids is carried out by means of a photoelectric densitometry. The method incorporates a procedure for eliminating variations in paper density. The results obtained are in good agreement with those obtained by ion exchange procedures.

Submitted on January 20, 1965
Accepted on October 20, 1965






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Copyright © 1966 by the American Association for Clinical Chemistry.