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Clinical Chemistry 12: 234-242, 1966;
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Clinical Chemistry, Vol 12, 234-242, Copyright © 1966 by the American Association for Clinical Chemistry

Direct Photometric Analysis of Serum Calcium with Glyoxal Bis (2-Hydroxyanil)

Milton Mager 1 and Genevieve Farese 1

1 Biochemistry Laboratory, U. S. Army Research Institute of Environmental Medi cine, Natick, Mass. 01762.

A spectrophotometric method for the direct determination of microgram quantities of calcium in serum is presented. In this procedure, 0.02 ml. of serum is interacted with buffered glyoxal bis (2-hydroxyanil) solution (GBHA) at pH 12.6. The resultant red calcium-GBHA complex is diluted in methanol-acetone-water solution, and the absorbance determined at 520 mµ. The effects of pH, temperature, GBHA concentration, and alcohol-water ratio were evaluated, as was interference by serum constituents. The method is simple and precise, and the analytical results compare well with the chloranilate procedure for serum calcium.

Submitted on November 19, 1965
Accepted on February 2, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.