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Clinical Chemistry 12: 274-281, 1966;
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Clinical Chemistry, Vol 12, 274-281, Copyright © 1966 by the American Association for Clinical Chemistry

The Automated Determination of NAD-Coupled Enzymes

II. Serum Lactate Dehydrogenase

Stanley Morgenstern 1, Richard Flor 1, Gerald Kessler, 1, and Bernard Klein 1

1 Automation Research Laboratory, Veterans Administration Hospital, and the Division of Neoplastic Disease, Montefiore Hospital, Bronx, N. Y.

A precise automated procedure developed for determination of serum lactate dehydrogenase, using the Robot Chemist, measures absorbance of a cuprous-neo-cuproine complex formed by coupled reduction of the cupric-neocuproine reagent with enzymatically generated reduced nicotinamide adenine dinucleotide. Activity values obtained by this method, by the identical automated colorimetric method on the AutoAnalyzer, and by an automated spectrophotometric (34O-mµ) procedure show excellent agreement.

Submitted on June 17, 1965
Accepted on December 27, 1965






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Copyright © 1966 by the American Association for Clinical Chemistry.