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Clinical Chemistry 12: 299-307, 1966;
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Clinical Chemistry, Vol 12, 299-307, Copyright © 1966 by the American Association for Clinical Chemistry

An Enzymatic-Spectrophotometric Determination of Pyruvic and Lactic Acid in Blood

Methodologic Aspects

J. C. Rosenberg 1 and B. F. Rush 1

1 Departments of Surgery, University of Kentucky Medical Center, Lexington, Ky., and Maumee Valley Hospital, Toledo, Ohio 43609.

Investigation of an enzymatic-spectrophotometric determination for lactic and pyruvic acid in blood has confirmed its suitability and reliability. The precision of both methods is high. No variability is encountered in the pyruvic acid analysis. Less than 5% variation is found with the lactic acid analysis. The recovery in both procedures approaches 100%.

Submitted on July 20, 1965
Accepted on January 10, 1966




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