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Clinical Chemistry 12: 308-313, 1966;
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Clinical Chemistry, Vol 12, 308-313, Copyright © 1966 by the American Association for Clinical Chemistry

A Rapid Electrophoretic Method for the Determination of the Isoenzymes of Serum Lactate Dehydrogenase

Albert W. Opher 1, Charles S. Collier 1, and Joseph M. Miller 1

1 General Medical and Surgical Research Service and Surgical Service, Veterans Administration Hospital. Fort Howard, Md., and Department of Surgery, School of Medicine, The Johns Hopkins University, Baltimore, Md.

A convenient electrophoretic procedure for the separation and quantitation of lactate dehydrogenase (LDH) isoenzymes is described. The system uses polyacetate Sepraphore III strips.* The areas of activity are shown by incubation with an LDH substrate combined with tetra-nitro-blue-tetrazolium. The reduction of the latter to the purple formazan is quantitatively related to the enzyme activity. Quantitative determination of the individual colored areas is performed by densitometry.

Submitted on January 12, 1965
Accepted on December 20, 1965






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Copyright © 1966 by the American Association for Clinical Chemistry.