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Clinical Chemistry 12: 406-413, 1966;
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Clinical Chemistry, Vol 12, 406-413, Copyright © 1966 by the American Association for Clinical Chemistry

An Automated Colorimetric (Tetrazolium Salt) Assay for Serum Lactate Dehydrogenase

Rodney D. Capps II 1, John G. Batsakis 1, Russell O. Briere 1, and Roger R. L. Calam 1

1 Departments of Pathology and Clinical Laboratories, The University of Michigan, 1335 E. Catherine St., Ann Arbor, Mich. 48104.

The adaptation of a tetrazolium salt assay for serum lactate dehydrogenase to fullautomation is described. The tetrazolium salt, INT, (2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride) serves as the chromogenic indicator of the enzyme activity. Phenazine methosulfate serves as an intermediate electron carrier.

Estimation requires only 5 min. of incubation, and assays may be run at the rate of 60/hr. Comparison against the manual version of the assay with respect to normal and abnormal serum activity levels is excellent.

Submitted on December 2, 1965
Accepted on April 18, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.