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Clinical Chemistry 12: 432-438, 1966;
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Clinical Chemistry, Vol 12, 432-438, Copyright © 1966 by the American Association for Clinical Chemistry

An Ultramicro Colorimetric Method for Determination of Total and Direct Serum Bilirubin

Roman B. Rutkowski 1 and Larissa deBaare 1

1 Department of Clinical Chemistry, WRAIR, Walter Reed Army Medical Center, Washington, D. C. 20012.

A simple reproducible method for the ultramicro determination of total and direct serum bilirubin is presented. This method provides a greater sensitivity than that obtained with other colorimetric ultramicro procedures. Total bilirubin values agree with those obtained with the macro Malloy-Evelyn procedure, but direct-reading bilirubin values were significantly higher with the presented method.

Submitted on August 23, 1965
Accepted on March 11, 1966






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Copyright © 1966 by the American Association for Clinical Chemistry.