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Clinical Chemistry 13: 985-993, 1967;
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Clinical Chemistry, Vol 13, 985-993, Copyright © 1967 by the American Association for Clinical Chemistry

Ultramicro Automated Screening Method for Uric Acid

Ronald H. Laessig 1, Chester E. Underwood 1, and Barbara J. Basteyns 1

1 University of Wisconsin, State Laboratory of Hygiene, Madison, Wis. 53706.

An automated colorimetric microprocedure, suitable for screening purposes, has been developed for the determination of blood uric acid levels. The method uses 2O-µl. whole-blood (capillary) samples and is based on the AutoAnalyzer measurement of the absorbance of the colored uric acid-phosphotungstic acid complex. The dilution inherent in the sampling procedure necessitated a modification of the existing AutoAnalyzer method to increase the sensitivity. The proposed method is evaluated for precision and accuracy by comparison with the standard AutoAnalyzer macro-method.

Submitted on December 22, 1966
Accepted on May 23, 1967







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Copyright © 1967 by the American Association for Clinical Chemistry.