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Clinical Chemistry 13: 227-232, 1967;
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Clinical Chemistry, Vol 13, 227-232, Copyright © 1967 by the American Association for Clinical Chemistry

Determination of Sodium in Urine by Specific Ion Electrode

Joseph S. Annino 1

1 University Hospital, Boston University Medical Center, Boston, Mass. 02118.

A method has been developed for determination of sodium concentration (activity) in urine using a glass electrode designed to be especially sensitive to sodium ion activity. The urine is diluted 2:20 with Tris buffer (pH 8.0) and sodium activity measured by dipping a sodium electrode and calomel reference electrode in the diluted specimen and obtaining a millivolt reading with a sensitive pH meter. The sodium concentration is then calculated from a predetermined standard curve. Concentrations of 0-200 mEq./L. may be read using the same dilution. Blind comparisons of sodium concentrations obtained by flame photometry and by sodium ion electrode showed excellent agreement.

Submitted on September 20, 1966
Accepted on November 1, 1966






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Copyright © 1967 by the American Association for Clinical Chemistry.