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Clinical Chemistry 13: 281-289, 1967;
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Clinical Chemistry, Vol 13, 281-289, Copyright © 1967 by the American Association for Clinical Chemistry

An Automated Alkaline Phosphatase Assay with Phenolphthalein Monophosphate as Substrate

Kirsten Hviid 1

1 Clinical Chemistry Laboratory, Peter Bent Brigham Hospital, Boston, Mass. 02115,

The manual procedure of Babson et al. (1) for the determination of serum alkaline phosphatase has been automated. The assay is based on the colorimetric determination of phenolphthalein formed on hydrolysis of phenolphthalein monophosphate. The procedure utilizes 0.16 ml. of serum without dialysis. Blanks are required only for turbid sera. Results are compared with those obtained by the manual procedure, and data relating to sample interaction, precision, blank values, and normal values are presented.

Submitted on September 30, 1966
Accepted on October 17, 1966






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Copyright © 1967 by the American Association for Clinical Chemistry.