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Clinical Chemistry 14: 1080-1090, 1968;
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Clinical Chemistry, Vol 14, 1080-1090, Copyright © 1968 by the American Association for Clinical Chemistry

The Colorimetric Estimation of Plasma Amino Nitrogen with DNFB

Jesse F. Goodwin 1

1 Clinical Research Center, Children’s Hospital of Michigan and Wayne State University School of Medicine, Detroit, Mich. 48202.

A simple procedure for the estimation of plasma amino nitrogen based on the reaction of amino groups with 2,4-dinitrofluorobenzene (DNFB) and subsequent measurement of the dinitrophenyl (DNP) amino derivatives at 420 mµ is presented. Elevated concentrations of bilirubin, hemoglobin, and the major nonprotein and nonamino acid nitrogen components of plasma do not seriously interfere. The proposed procedure compares favorably with a corresponding ninhydrin technic for plasma amino nitrogen. The mean plasma amino nitrogen values and range for a group of adults (age 18-50 years) by the proposed method is 5.56 mg./100 ml. (3.6-7.0 mg./100 ml.) compared to 4.12 mg./100 ml. (3.6-5.2 mg./100 ml.) for a group of children (age 2-10 years). Values from a group of children with sickle cell anemia showed no significant variance with the normal population. Optimum conditions for quantitation of plasma amino nitrogen along with other procedural parameters are presented.

Submitted on February 17, 1968
Accepted on March 14, 1968




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Copyright © 1968 by the American Association for Clinical Chemistry.