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Clinical Chemistry 17: 988-991, 1971;
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Clinical Chemistry, Vol 17, 988-991, Copyright © 1971 by the American Association for Clinical Chemistry

Evaluation of Light-Scattering Index (Nephelometry) for Assessing Serum Triglycerides and Lipoprotein Phenotypes

Edith Zak Helman 1, Eleanor J. Blevins 1, and Irene O. Gleason 1

1 Veterans Administration Hospital, Long Beach, Calif. 90801.

Light-scattering measurements (nephelometry) of serum after filtration through appropriate filters is a rapid method for physically determining triglyceride concentrations and lipoprotein phenotypes. A correlation coefficient of greater than 0.9 was obtained when aliquots of the same specimen were tested for triglycerides by light scattering in an Aminco-Bowman Spectrophotofluorometer and by a chemical (Van Handel and Zilversmit) method. Lipoprotein phenotyping by agarose gel electrophoresis and by light-scattering measurement corresponded excellently. In equivocal cases, nephelometric results agreed better with the triglyceride and cholesterol data than did the electrophoretic method.


Key Words: colorimetry-nephelometry relationships • Fredrickson’s phenotypes

Submitted on March 11, 1971
Accepted on June 18, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.