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Clinical Chemistry 17: 1180-1182, 1971;
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Clinical Chemistry, Vol 17, 1180-1182, Copyright © 1971 by the American Association for Clinical Chemistry

Automated Procedure for Determination of Bilirubin in Human Sera

J. D. H. Cooper 1

1 Department of Biochemistry, Coventry and Warwickshire Hospital, Coventry, U.K.

An automated procedure is presented, based on the method of Bruchner [Amer. J. Clin. Pathol. 32, 513 (1959)], for determination of both total and conjugated bilirubin. The method is standardized by use of bilirubin in human serum, which is stabilized by a mixture of formamide and cyanide. Hemoglobin does not interfere at concentrations less than 500 mg/100 ml, and the effect of lipemia is minimized.


Key Words: formamide—cyanide stabilizer • AutoAnalyzer • spectrophotometry of azo derivative • hemoglobin and lipemia, interference by

Submitted on July 22, 1971
Accepted on September 15, 1971






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Copyright © 1971 by the American Association for Clinical Chemistry.