Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 17: 400-402, 1971;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ceriotti, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ceriotti, G.

Clinical Chemistry, Vol 17, 400-402, Copyright © 1971 by the American Association for Clinical Chemistry

Ultramicrodetermination of Plasma Urea by Reaction with Diacetylmonoxime—Antipyrine without Deproteinization

Giovanni Ceriotti 1

1 Laboratorio Centrale di Analisi, Ospedale Civile di Padova, Padua, Italy.

Urea can be directly determined in serum or plasma by reaction with diacetylmonoxime—antipyrine: 5 µl of the sample is mixed with 5 ml of the chromogenic reagent, and the mixture is boiled 15 min, cooled, and read at 460 nm. The color is stable for 30 min in the light. The reagent is composed of (a) 0.4 g of antipyrine per 100 ml of H2SO4 (20 ml/100 ml), containing 5 mg of ferric sulfate per 100 ml: 1 (or 2) volume(s), and (b) 0.5 g of diacetylmonoxime per 100 ml of acetic acid (5 ml/100 ml): 1 volume.


Key Words: AutoAnalyzer

Submitted on November 23, 1970
Accepted on February 4, 1971




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Appl. Physiol.Home page
W. Derave, B. Marescau, E. V. Eede, B. O. Eijnde, P. P. De Deyn, and P. Hespel
Plasma guanidino compounds are altered by oral creatine supplementation in healthy humans
J Appl Physiol, September 1, 2004; 97(3): 852 - 857.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
W. J de Jonge, M. M Hallemeesch, K. L Kwikkers, J. M Ruijter, C. de Gier-de Vries, M. A van Roon, A. J Meijer, B. Marescau, P. P De Deyn, N. E. Deutz, et al.
Overexpression of arginase I in enterocytes of transgenic mice elicits a selective arginine deficiency and affects skin, muscle, and lymphoid development
Am. J. Clinical Nutrition, July 1, 2002; 76(1): 128 - 140.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
W. J. de Jonge, B. Marescau, R. D'Hooge, P. P. De Deyn, M. M. Hallemeesch, N. E. P. Deutz, J. M. Ruijter, and W. H Lamers
Overexpression of Arginase Alters Circulating and Tissue Amino Acids and Guanidino Compounds and Affects Neuromotor Behavior in Mice
J. Nutr., October 1, 2001; 131(10): 2732 - 2740.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1971 by the American Association for Clinical Chemistry.