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Clinical Chemistry 17: 529-534, 1971;
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Clinical Chemistry, Vol 17, 529-534, Copyright © 1971 by the American Association for Clinical Chemistry

Simplified Manual Micromethod for Determination of Serum Triglycerides

Felix G. Soloni 1

1 Lipid Laboratory, DADE Division American Hospital Supply Corp., 1851 Delaware Parkway, Miami, Fla. 33152.

This rapid, simplified assay for serum triglycerides is based on the use of a more effective solvent extraction procedure, ethoxide transesterification, and color development with acetylacetone. No phospholipid adsorption step is necessary. The procedure requires less than 0.2 ml of serum, no expensive or elaborate apparatus, and 5 to 10 values can be obtained per hour, with better precision than with most existing methods. Recovery averaged 99.9%. There are practically no interferences. Results correlated excellently with those obtained by the classical Carlson-Wadström method.


Key Words: spectrophotometry • fluorometry • alkoxide transesterification • acetylacetone color reaction • normal values • Carlson-Wadström procedure compared

Submitted on January 29, 1971
Accepted on April 7, 1971




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Copyright © 1971 by the American Association for Clinical Chemistry.