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Clinical Chemistry 18: 895-900, 1972;
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Clinical Chemistry, Vol 18, 895-900, Copyright © 1972 by the American Association for Clinical Chemistry

Negative Interference by Residual Proteins in the Supernatant Fluid Used in Radioisotopic Assay of Serum Vitamin B12

Esper Mortensen 1

1 Department of Clinical Chemistry, Kommunehospitalet, 8000 Århus C, Denmark.

Separation of vitamin B12, from serum by heat denaturation results in an individually variable amount of residual protein in the supernatant fluid. I show that during radioisotopic assay of serum vitamin B12, such protein may seriously interfere with the results, and that the interference may be abolished by changing the experimental conditions.


Key Words: intrinsic factor binding capacity • separation of vitamin B12 by heat denaturation of serum • bound and unbound B12 • protein-coated charcoal reagent • binding kinetics

Submitted on May 1, 1972
Accepted on May 18, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.