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Clinical Chemistry 18: 934-936, 1972;
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Clinical Chemistry, Vol 18, 934-936, Copyright © 1972 by the American Association for Clinical Chemistry

Spot Test for Detection of Sickling Hemoglobin

Sally Kelly 1 and Lucille Desjardins 1

1 New York State Department of Health, Birth Defects Institute, New Scotland Ave., Albany, N.Y. 12201.

We designed a simple spot-plate method for detecting sickling hemoglobins and distinguishing hemoglobin SS from AS in samples of dried blood on filter paper. The test depends on the differential solubility of sickling hemoglobins in phosphate solutions with and without 3 molar urea. Paired phosphate and phosphate-urea hemolysates form color combinations that match those from control hemoglobins.

Submitted on April 10, 1972
Accepted on May 8, 1972






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Copyright © 1972 by the American Association for Clinical Chemistry.