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Clinical Chemistry 19: 1384-1386, 1973;
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Clinical Chemistry, Vol 19, 1384-1386, Copyright © 1973 by the American Association for Clinical Chemistry

Modified Automated Saccharogenic Determination of Serum and Urinary Amylase Activity

W. S. Matthews 1, R. E. Sterling 1, T. Boyd 1, and O. R. Flores 1

1 Department of Pathology, Los Angeles County-University of Southern California Medical Center, 1200 N. State St., Los Angeles, Calif. 90033.

The automated method of O’Neal and Gochman [Clin. Chem. 16, 985 (1970)] for determination of serum and urine amylase activity has been modified to increase its accuracy, precision, and efficiency. Sampling rate is 50 specimens per hour. The analysis depends on agr-amylase hydrolyzing glycogen to maltose-like subunits, which are measured with the copper neocuproine reaction. Results obtained by this method are compared to those for a manual reference method [Clin. Chem. 6, 434 (1960)].






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Copyright © 1973 by the American Association for Clinical Chemistry.