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Clinical Chemistry 19: 937-941, 1973;
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Clinical Chemistry, Vol 19, 937-941, Copyright © 1973 by the American Association for Clinical Chemistry

Automated Reaction-Rate Method for the Direct Determination of Total Serum Cholesterol by Use of the "CentrifiChem" Parallel Fast Analyzer

K. A. Slickers 1, L Edwards 1, J. Daly 1, and G. Ertingshausen 1

1 Erie County Laboratories and the State University of New York at Buffalo, 462 Grider St., Buffalo, N. Y. 14215 (K. A. S. and L. E.); and the Union Carbide Research Institute, Tarrytown, N.Y. 10591 (J. D. and G. E.).

The direct procedure for determining serum cholesterol described by Wybenga et al. [Clin. Chem. 16, 980 (1970)] can be used to assay 29 samples in 10-min reaction time by using the "CentrifiChem" parallel fast analyzer (Union Carbide). Commercial reference serum was used for standardization. Correlation of results with those obtained by the method of Abell et al. [J. Biol. Chem. 195, 357 (1952)] for 54 samples yielded a regression line with a slope (m) of 0.897 and a y-intercept (b) of 231 mg/liter; the correlation coefficient (r) was 0.971. Compared with the manual procedure of Parekh and Jung [Anal. Chem. 42, 1423 (1970)] for 146 samples, the corresponding values were m = 0.991, b = 27 mg/ liter, and r = 0.981. Compared to serum extracts assayed with the "AutoAnalyzer" (Standard Technicon Methodology N-24a), 186 samples gave corresponding values of m = 0.990, b = 92 mg/ liter, and r = 0.979. No bias significant at the 95% confidence level existed versus any of the three methods. Sera containing abnormally high bilirubin concentrations (>50 mg/liter) or abnormally high triglyceride content (>5,000 mg/liter) did not give significantly different cholesterol values on the CentrifiChem as compared with values obtained with either the AutoAnalyzer or the Parekh and Jung method.


Key Words: AutoAnalyzer • Abell—Kendall method • Parekh—Jung method

Submitted on March 23, 1973
Accepted on April 27, 1973






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Copyright © 1973 by the American Association for Clinical Chemistry.